DetoxFungiV1, Main, Exploration, bibRecord, 001819

Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics.

Identifieur interne : 001819 ( Main/Exploration ); précédent : 001818; suivant : 001820

Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics.

Auteurs : Wataru Aoki [Japon] ; Yohei Tatsukami ; Nao Kitahara ; Kazuma Matsui ; Hironobu Morisaka ; Kouichi Kuroda ; Mitsuyoshi Ueda

Source :

Journal of proteomics [ 1876-7737 ] ; 2013.

RBID : pubmed:23948566

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Candida albicans (metabolism), Candidiasis (microbiology), Cattle (MeSH), Cluster Analysis (MeSH), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Fungal (MeSH), Molecular Sequence Data (MeSH), Open Reading Frames (MeSH), Protein Structure, Secondary (MeSH), Proteomics (MeSH), Sequence Homology, Amino Acid (MeSH), Serum (metabolism), Serum (microbiology), Time Factors (MeSH), Virulence Factors (metabolism).
MESH :
- chemical , metabolism : Fungal Proteins, Virulence Factors.
- metabolism : Candida albicans, Serum.
- microbiology : Candidiasis, Serum.
- Amino Acid Sequence, Animals, Cattle, Cluster Analysis, Gene Expression Profiling, Gene Expression Regulation, Fungal, Molecular Sequence Data, Open Reading Frames, Protein Structure, Secondary, Proteomics, Sequence Homology, Amino Acid, Time Factors.

Abstract

UNLABELLED

Candida albicans is an opportunistic pathogen that causes fatal disease if the host immunity is compromised. The mortality rate of systemic candidiasis is very high; hence, there is a ceaseless demand for novel pharmaceuticals. In this study, quantitative time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) is described. Survival in blood is essential for virulence of C. albicans, and a detailed analysis is required. We cultivated C. albicans in FBS for 0-180min, and determined quantitative time-course variations of 1024 proteins in the cultured cells by using a LC-MS/MS system with a long monolithic silica capillary column. Clustering analysis identified FBS-induced proteins associated with detoxification of oxidative species, high-affinity glucose transport, citrate cycle, oxidative phosphorylation, and iron acquisition. Furthermore, we identified possible virulence factors such as orf19.4914.1 (named Blood-induced peptide 1, Blp1). Heterologous expression of BLP1 in Saccharomyces cerevisiae shortened the lag phase and resulted in a pleiotropic stress-tolerance phenotype, indicating a possible role for quick adaptation to a stressful environment. While further experiments are necessary to prove virulence of the identified factors, systematic identification of candidate virulence proteins in this study will lead to profound understanding of virulence of C. albicans.

BIOLOGICAL SIGNIFICANCE

This paper describes time-course proteomics of C. albicans during adaptation to serum, which is an essential process for fatal systemic candidiasis. Using a LC-MS/MS system with a monolithic silica capillary column, we have successfully characterized time-course variations of 1024 proteins. Among them, orf19.4914.1 (Blp1) was identified as a novel pleiotropic stress-tolerance peptide, which could have an important role for virulence of C. albicans.

DOI: 10.1016/j.jprot.2013.07.031
PubMed: 23948566

Affiliations:

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Le document en format XML

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<term>Animals (MeSH)</term>
<term>Candida albicans (metabolism)</term>
<term>Candidiasis (microbiology)</term>
<term>Cattle (MeSH)</term>
<term>Cluster Analysis (MeSH)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Open Reading Frames (MeSH)</term>
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<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Serum (metabolism)</term>
<term>Serum (microbiology)</term>
<term>Time Factors (MeSH)</term>
<term>Virulence Factors (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Analyse de regroupements (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Bovins (MeSH)</term>
<term>Cadres ouverts de lecture (MeSH)</term>
<term>Candida albicans (métabolisme)</term>
<term>Candidose (microbiologie)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Facteurs de virulence (métabolisme)</term>
<term>Facteurs temps (MeSH)</term>
<term>Protéines fongiques (métabolisme)</term>
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<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Structure secondaire des protéines (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Sérum (microbiologie)</term>
<term>Sérum (métabolisme)</term>
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<term>Gene Expression Regulation, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Open Reading Frames</term>
<term>Protein Structure, Secondary</term>
<term>Proteomics</term>
<term>Sequence Homology, Amino Acid</term>
<term>Time Factors</term>
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<term>Analyse de regroupements</term>
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<term>Bovins</term>
<term>Cadres ouverts de lecture</term>
<term>Données de séquences moléculaires</term>
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<term>Régulation de l'expression des gènes fongiques</term>
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<front><div type="abstract" xml:lang="en"><p><b>UNLABELLED</b>
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<p>Candida albicans is an opportunistic pathogen that causes fatal disease if the host immunity is compromised. The mortality rate of systemic candidiasis is very high; hence, there is a ceaseless demand for novel pharmaceuticals. In this study, quantitative time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) is described. Survival in blood is essential for virulence of C. albicans, and a detailed analysis is required. We cultivated C. albicans in FBS for 0-180min, and determined quantitative time-course variations of 1024 proteins in the cultured cells by using a LC-MS/MS system with a long monolithic silica capillary column. Clustering analysis identified FBS-induced proteins associated with detoxification of oxidative species, high-affinity glucose transport, citrate cycle, oxidative phosphorylation, and iron acquisition. Furthermore, we identified possible virulence factors such as orf19.4914.1 (named Blood-induced peptide 1, Blp1). Heterologous expression of BLP1 in Saccharomyces cerevisiae shortened the lag phase and resulted in a pleiotropic stress-tolerance phenotype, indicating a possible role for quick adaptation to a stressful environment. While further experiments are necessary to prove virulence of the identified factors, systematic identification of candidate virulence proteins in this study will lead to profound understanding of virulence of C. albicans.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>BIOLOGICAL SIGNIFICANCE</b>
</p>
<p>This paper describes time-course proteomics of C. albicans during adaptation to serum, which is an essential process for fatal systemic candidiasis. Using a LC-MS/MS system with a monolithic silica capillary column, we have successfully characterized time-course variations of 1024 proteins. Among them, orf19.4914.1 (Blp1) was identified as a novel pleiotropic stress-tolerance peptide, which could have an important role for virulence of C. albicans.</p>
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<Abstract><AbstractText Label="UNLABELLED">Candida albicans is an opportunistic pathogen that causes fatal disease if the host immunity is compromised. The mortality rate of systemic candidiasis is very high; hence, there is a ceaseless demand for novel pharmaceuticals. In this study, quantitative time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) is described. Survival in blood is essential for virulence of C. albicans, and a detailed analysis is required. We cultivated C. albicans in FBS for 0-180min, and determined quantitative time-course variations of 1024 proteins in the cultured cells by using a LC-MS/MS system with a long monolithic silica capillary column. Clustering analysis identified FBS-induced proteins associated with detoxification of oxidative species, high-affinity glucose transport, citrate cycle, oxidative phosphorylation, and iron acquisition. Furthermore, we identified possible virulence factors such as orf19.4914.1 (named Blood-induced peptide 1, Blp1). Heterologous expression of BLP1 in Saccharomyces cerevisiae shortened the lag phase and resulted in a pleiotropic stress-tolerance phenotype, indicating a possible role for quick adaptation to a stressful environment. While further experiments are necessary to prove virulence of the identified factors, systematic identification of candidate virulence proteins in this study will lead to profound understanding of virulence of C. albicans.</AbstractText>
<AbstractText Label="BIOLOGICAL SIGNIFICANCE" NlmCategory="UNASSIGNED">This paper describes time-course proteomics of C. albicans during adaptation to serum, which is an essential process for fatal systemic candidiasis. Using a LC-MS/MS system with a monolithic silica capillary column, we have successfully characterized time-course variations of 1024 proteins. Among them, orf19.4914.1 (Blp1) was identified as a novel pleiotropic stress-tolerance peptide, which could have an important role for virulence of C. albicans.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aoki</LastName>
<ForeName>Wataru</ForeName>
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<AffiliationInfo><Affiliation>Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.</Affiliation>
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<Keyword MajorTopicYN="N">MS/MS</Keyword>
<Keyword MajorTopicYN="N">Quantitative proteome</Keyword>
<Keyword MajorTopicYN="N">S. cerevisiae</Keyword>
<Keyword MajorTopicYN="N">SD+HLM</Keyword>
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<Keyword MajorTopicYN="N">TEAB</Keyword>
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<Keyword MajorTopicYN="N">synthetic dextrose+histidine, leucine, methionine</Keyword>
<Keyword MajorTopicYN="N">tandem mass spectrometry</Keyword>
<Keyword MajorTopicYN="N">tandem mass tag</Keyword>
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<tree><noCountry><name sortKey="Kitahara, Nao" sort="Kitahara, Nao" uniqKey="Kitahara N" first="Nao" last="Kitahara">Nao Kitahara</name>
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<name sortKey="Tatsukami, Yohei" sort="Tatsukami, Yohei" uniqKey="Tatsukami Y" first="Yohei" last="Tatsukami">Yohei Tatsukami</name>
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   |texte=   Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics.
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Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics.

Elucidation of potentially virulent factors of Candida albicans during serum adaptation by using quantitative time-course proteomics.

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